- Prepare a 30 mL or more sample of particles in liquid suspension. Particle density should be between 105 and 107 particles/mL for most acccurate results.
- Start the Particle Insight application. Create a new sample file using New. Check and adjust settings:
- Analysis Conditions control how images are analyzed.
- Run Conditions determine run length and operations during and after the run, such as image saving or individual particle data saving.
- Report and Display Options relate to the user interface and data reporting; they do not affect the results and can be modified later if needed.
- Pour the sample into the sample well and activate the pump. Use Single, Continuous, and single image Analyze to check image quality and particle density.
- Open any desired measure windows, so as to monitor those measures as the run proceeds.
- Open System Performance Data to monitor run control parameters such as image and particle counts.
- Click Clear and Start to begin the run. The run will end at the preset limits, and will be auto-saved if that option is on. A typical run of 20,000 particles will take 15 to 45 seconds depending on particle density.
- Use Print to output run results to a printer or PDF file. Set report options in Report and Display Options.